Fig. 2. Viability of 4T1 cells cultured in different amounts of RAW 264.7 macrophage-conditioned media: non-treated (CM-NT) (A) or PBP-treated (CM-PBP) (B). The cells were cultured in CM for 24, 48 and 72 h. Cell viability was measured using the MTT assay. The reported data are the means ± SEM of three independent experiments with six wells for each experiment. Viability of the cells is presented as the percentage of cells incubated in complete DMEM medium containing 10, 25, 50 or 75% culture medium supplemented with 1% FBS (control group). Asterisks show significant differences between the cells cultured in CM-NT or CM-PBP compared with 0% conditioned-medium (***P<0.001; **P<0.01; *P<0.05).